Diversity oriented one-pot three-component sequential synthesis of annulated benzothiazoloquinazolines
© Kumar et al; licensee Springer. 2012
Received: 29 September 2011
Accepted: 2 March 2012
Published: 2 March 2012
Annulated benzothiazoloquinazolines have been synthesized by a diversity oriented simple and convenient synthesis involving one-pot three-component reaction of substituted 2-aminobenzothiazoles with α-tetralone and aromatic/heteroaromatic aldehydes in ethanol in the presence of catalytic amount of triethylamine. The synthesized compounds have been characterized by their elemental analyses and spectral data.
Keywordsbenzothiazoloquinazolines one-pot 2-aminobenzothiazoles cyclic ketones thiophene-2-carbaldehyde.
The synthesis of fused heterocycles has attracted considerable interest in heterocyclic chemistry as the fusion of biodynamic heterosystems has proved to be a very attractive and useful for the design of new molecular framework of potential drugs with varying pharmacological activities. A major challenge of modern drug discovery is to design highly efficient chemical reaction sequences which provide molecules containing maximum complexity and structural diversity with interesting bioactivities in minimum number of synthetic steps. Recently, multicomponent reactions have attracted attention of chemical and pharmaceutical research and emerged as highly efficient and synthetically useful reactions for the preparation of structurally diverse drug- like heterocyclic compounds.
There has been an increasing interest in the chemistry of quinazolines [1–5] because of being present quinazoline heterocyclic system as a building block in many natural and synthetic products capable of exhibiting a wide variety of biological and pharmacological activities . Quinazolines have been reported to exhibit anti-inflammatory , antihypertensive , anticancer , antitumor  and antibacterial  activities. Quinazoline and its derivatives have recently been evaluated as antagonist of various biological receptors such as 5-HT5A  related disease calcitonin gene - related peptide  and vasopressin V3 receptors . Benzothiazole has also been an interesting heterocyclic system in drug research on account of significant biological activities of its fused derivatives [15–17]. Thiazoloquinazolines, have shown significant activity against cancer . Thiazoloquinazolines have also been identified as cyclin dependent kinase (CDK) and glycogen synthase kinase (GSK-3) inhibitors [19, 20].
Result and discussion
Reaction of 2-aminobenzothiazoles with α-tetralone and aromatic/heteroaromatic aldehydes.
The structures of the synthesized compounds were confirmed by their elemental analyses and spectral data. Infrared spectra of all the synthesized compounds exhibit an intense unsaturation absorption band in the region 1605-1610 cm-1 (C = C). The absorption band in the region 1620-1654 cm-1 in the IR spectra of all the compounds indicated the presence of C = N bond. Two absorption bands corresponding to asymmetric and symmetric stretching vibrations of -NH2 group, which were present in the IR spectra of 2-aminobenzothiazoles, are absent in the IR spectra of the synthesized compounds. 1H NMR spectra of the synthesized compounds showed a multiplet in the region δ 7.01-8.08 ppm due to aromatic protons. The singlet appeared in the region δ 5.90-6.80 was assigned to the aliphatic proton (C7-H). The singlet observed in the region δ 3.69-3.84 ppm was attributed to the methoxy protons, whereas the methyl protons resonate as a singlet in the region δ 2.34-2.36 ppm in the 1H NMR spectra of annulated benzothiazoloquinazolines containing -OCH3 and -CH3 groups respectively. The multiplets observed in the region δ 2.74-3.23 ppm were assigned to the methylene protons at C-5 and C-6. In the 13C NMR spectra of the synthesized annulated benzothiazoloquinazolines, the δ values of most of the carbon atoms of CH3, CH2, CH, C = C, C = N, C-N, C-O, C-S, C-F and aromatic system could be determined by distinct resonance signals and were found to be in agreement with the proposed structures.
In conclusion, we have developed a simple and convenient diversity oriented one-pot three-component sequential synthesis for the synthesis of structurally diverse heterocycles, annulated benzothiazoloquinazolines, incorporating medicinally privileged heterosystems.
The present method with its mild reaction conditions and operational simplicity enables the sequential combination of three reactive components; α-tetralone, thiophene-2-carbaldehyde/p-methoxybenzaldehyde and 2-aminobenzothiazole in one-pot and efficiently incorporates structural diversity simply by varying the substituents or by slight structural modification in the components involved in the reaction. This new method has the advantages of higher yields, mild reaction conditions, shorter reaction time, and convenient procedure and can be extended to prepare a library of structurally diverse drug like small heterocyclic molecules.
Melting points were determined on an electric melting point apparatus and are uncorrected. The purity of all the compounds was checked by thin layer chromatography using various non-aqueous solvents. IR spectra (KBr pellets) were recorded on Shimadzu-8400S FT-IR Spectrophotometer. The 1H NMR and 13C NMR spectra were scanned on Jeol AL 300 FT NMR in CDCl3 using TMS as an internal standard. The chemical shifts are expressed as δ ppm.
Procedure for the preparation of 7-thien-2-yl/4'-methoxyphenyl-5, 6-dihydro-7H-benzo [h] benzothiazolo [2, 3-b] quinazolines
To a magnetically stirred solution of thiophene-2-carbaldehyde/p-methoxy benzaldehyde (0.01 mole) and α-tetralone (0.01 mole) in ethanol (20 ml), a catalytic amount of (C2H5)3N was added slowly and further stirred for 30 min. Then 2-aminobenzothiazole (0.01 mole) was added to the reaction mixture with stirring for 2 h and then refluxed for 60-90 min in a round bottom flask fitted with reflux condenser. The progress of the reaction was monitored by TLC. The precipitate formed after the completion of reaction was isolated by filtration. The solid separated out was washed well with ethanol, dried and finally crystallized from ethanol.
11-Methyl-7-(thien-2-yl)-5,6-dihydro-7H-benzo[h]benzothiazolo[2, 3-b]quinazoline (4a)
(4a). m.p. 210-212°C; yield: 76%; IR (KBr, cm-1): 3100, 2980, 1620, 1605; 1H NMR (300 MHz, CDCl3) δppm: 7.10-7.68 (m, 10H, Ar-H), 5.86 (s, 1H, C7), 3.18-3.23 (m, 2H, CH2), 2.93-3.05 (m, 2H, CH2), 2.36 (s, 3H, CH3); 13C NMR (75 MHz, CDCl3) δppm: 18.38, 26.98, 28.10, 58.32, 119.43, 122.13, 125.34, 125.53, 126.23,126.56, 126.85, 127.80, 127.32, 127.54, 128.26, 130.20, 132.14, 133.65, 134.12, 137.34, 139.14,143.15, 167.35; Anal. Calcd. for C23H18N2S2: C, 71.47; H, 4.69; N, 7.25; Found: C, 73.65; H, 4.99; N, 7.19.
11-Methoxy-7-(thien-2-yl)-5,6-dihydro-7H-benzo[h]benzothiazolo [2, 3-b] quinazoline (4b)
(4b) m.p. 220-222°C; yield: 80%, IR (KBr, cm-1): 3010-3080, 2975, 1620, 1615; 1H NMR (300 MHz, CDCl3) δppm: 7.09-7.84 (m, 10H, Ar-H), 5.98 (s, 1H,C7-H), δ 3.84 (s, 3H, OCH3), 2.96-3.22 (m, 4H, CH2, C5, and C6); 13C NMR (75 MHz, CDCl3) δppm: 26.9, 28.06, 54.60, 57.20, 115.70, 122.52, 125.29, 125.62, 126.14, 126.45, 127.58, 127.69, 128.08, 129.44, 130.68, 133.12, 133.28, 134.60, 137.20, 139.04, 142.98, 155.61, 167.19 Anal. Calcd. for C23H18N2OS2: C, 68.63; H, 4.51; N, 6.96; Found: C, 70.65; H, 4.35; N, 6.99.
9,10-Dichloro-7-(thien-2-yl)-5,6-dihydro-7H-benzo[h]benzothiazolo-[2, 3-b]quinazoline (4c)
(4c) m.p. 205-207°C; yield: 77%, IR (KBr, cm-1): 3060, 2972, 1622, 1615; 1H NMR (300 MHz, CDCl3) δppm: 7.08-7.45 (m, 9H, Ar-H), 5.96 (s, 1H,C7-H), δ 3.12-3.24 (m, 2H,CH2) 2.94-2.96 (m, 2H,CH2); 13C NMR (75 MHz, CDCl3) δppm: 26.96, 28.16, 57.23, 119.92, 120.32, 123.46, 125.13, 125.24, 126.23, 126.52, 127.26, 127.46, 128.13, 130.32, 131.82, 133.65, 134.91, 138.03, 139.45, 143.98, 167.16; Anal. Calcd. for C22H14Cl2N2S2: C, 59.86; H, 3.20; N, 6.35; Found: C, 62.09; H, 3.10; N, 6.45.
11-Fluoro-7-(thien-2-yl)-5,6-dihydro-7H-benzo[h]benzothiazolo-[2, 3-b]quinazoline (4d)
(4d) m.p. 190-192°C; yield: 75%, IR (KBr, cm-1): 3075, 2978, 1620, 1610; 1H NMR (300 MHz, CDCl3) δ ppm: 6.92-7.40 (m, 10H, Ar-H), 5.95 (s, 1H, C7-H), δ 3.18-3.23 (m, 2H,CH2) 3.01-3.05 (m, 2H, CH2); 13C NMR (75 MHz, CDCl3) δppm: 26.78, 28.13, 58.62, 119.23, 120.35, 122.16, 125.28, 125.69, 126.31, 126.65, 127.58, 127.67, 128.23, 131.98, 133.54, 134.46, 138.42, 139.68, 142.06, 153.72, 170.43; Anal. Calcd. for C22H15FN2S2: C, 67.67; H, 3.87; N, 7.17; Found: C, 67.65; H, 3.83; N, 7.13.
11-Methyl-7-(4-methoxyphenyl)-5,6-dihydro-7H-benzo[h]benzothiazolo[2, 3-b]quinazoline (4e)
(4e) m.p. 215-217°C; yield: 80%, IR (KBr, cm-1): 3045, 2977, 1650, 1610,1275 1H NMR (300 MHz, CDCl3) δppm: 7.20-7.54 (m, 11H, Ar-H), 5.90 (s, 1H, C7-H), 3.76 (s, 3H, OCH3), 2.94-3.01 (m, 2H, CH2), 2.80-2.86 (m, 2H, CH2), 2.26 (s, 3H, CH3); 13C NMR (75 MHz, CDCl3) δppm: 18.26, 26.98, 28.68, 54.92, 57.23, 113.74, 114.96, 115.26, 123.64, 125.32, 125.76, 126.23, 126.64, 127.19, 127.84, 128.68, 128.96, 130.05, 132.92, 133.30, 134.52, 143.42, 156.39, 169.30; Anal. Calcd. for C26H22N2OS: C, 76.07; H, 5.40; N, 6.82; Found: C, 76.01; H, 5.15; N, 6.92.
11-Methoxy-7-(4-methoxyphenyl)-5,6-dihydro-7H-benzo[h]benzothiazolo-[2, 3-b]quinazoline (4f)
(4f) m.p. 227-229°C; yield: 80%, IR (KBr, cm-1): 3100, 3080-3050, 1648, 1612; 1H NMR (300 MHz, CDCl3) δppm: 7.15-7.52 (m, 11H, Ar-H), 5.90 (s, 1H, C7-H), δ 3.73 (s, 3H, OCH3), 3.69 (s, 3H, OCH3), 2.94-3.00 (m, 2H, CH2), 2.80-2.86 (m, 2H, CH2); 13C NMR (75 MHz, CDCl3) δppm: 28.26, 28.95, 54.20, 55.09, 56.96, 115.52, 117.52, 119.32, 120.44, 125.18, 126.48, 127.32, 127.47, 127.80, 128.12, 128.76, 133.07, 133.40, 134.23, 136.35, 137.98, 153.62, 158.26, 169.39; Anal. Calcd. for C26H22N2O2S: C, 76.2; H, 5.2; N, 6.86; Found: C, 76.07; H, 5.40; N, 6.82.
9,10-Dichloro-7-(4-methoxyphenyl)-5,6-dihydro-7H-benzo[h]benzothiazolo[2, 3-b]quinazoline (4g)
(4g) m.p. 192-194°C; yield: 74%, IR (KBr, cm-1): 3008-3050, 2972, 1645, 1612, 1270; 1H NMR (300 MHz, CDCl3) δppm: 7.12-7.54 (m, 10H, Ar-H), 5.92 (s, 1H, C7-H), δ 3.73 (s, 3H,OCH3), 2.74-2.99 (m, 4H, CH2 at C5 and C6); 13C NMR (75 MHz, CDCl3) δppm: 26.98, 28.12, 55.08, 58.74, 115.26, 115.35, 119.62, 120.43, 125.63, 127.48, 127.32, 127.58, 128.11, 128.45, 128.69, 130.24, 131.67, 133.40, 133.86, 134.26, 144.53, 155.62, 167.38; Anal. Calcd. for C25H18Cl2N2OS: C, 64.49; H, 3.88; N, 6.06; Found: C, 64.52; H, 3.90; N, 6.02.
11-Fluoro-7-(4-methoxyphenyl)-5,6-dihydro-7H-benzo[h]benzothiazolo-[2, 3-b]quinazoline (4h)
(4h) m.p. 189-191°C; yield: 75%, IR(KBr, cm-1): 3010-3072, 2974, 1648, 1612, 1242; 1H NMR (300 MHz, CDCl3) δppm: 7.12-7.54 (m, 11H, Ar-H), 5.95 (s, 1H, C7-H), δ 3.69 (s, 3H, OCH3), 2.82-2.96 (m, 4H, CH2 at C5 and C6); 13C NMR (75 MHz, CDCl3) δppm: 26.98, 28.56, 55.63, 57.39, 115.47, 118.78, 119.12, 121.45, 125.73, 126.63, 127.32, 127.58, 128.16, 128.73, 128.97, 131.43, 133.26, 133.89, 134.38, 142.19, 153.76, 170.43; Anal. Calcd. for C25H19FN2OS: C, 72.40; H, 4.60; N, 6.72; Found: C, 72.44; H, 4.62; N, 6.76.
The authors were thankful to the Head, Department of Chemistry, University of Rajasthan, Jaipur for providing laboratory and instrumentation facilities. RSIC, CDRI, Lucknow is also acknowledged for providing analytical and spectral data of the compounds. One of the authors (KS) thanks University of Rajasthan Jaipur (India) for the research fellowship.
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