Organic and Medicinal Chemistry Letters

Table 1 Phosphorylation of N^β-methylated peptidomimetic substrates by PKA catalytic subunit at ATP concentration 100 μM, 30°C, 50 mM TRIS/HCl, pH 7

From: N^β-methylation changes the recognition pattern of aza-β³-amino acid containing peptidomimetic substrates by protein kinase A

	Substrate	K _m (μM)	10² k _cat (μmole mg^-1 s^-1)	10² k _II (L mg^-1 s^-1)
I	R RASVA	209 ± 24	24.4 ± 1.5	0.098 ± 0.010
II	RR ASVA	79 ± 18	11.9 ± 1.0	0.11 ± 0.07
III	RRA SVA	91 ± 15	5.5 ± 0.3	0.034 ± 0.005
IV	RRASV A	118 ± 36	16 ± 2.0	0.12 ± 0.02
V	RRASVA	57 ± 17	5.0 ± 0.4	0.090 ± 0.015
	RRASVA (from [16])	11.1 ± 3.5	36 ± 3	3.2 ± 0.1

AA, N^β-Me-aza-β³-amino acid.

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