FT-IR study of the polysaccharides isolated from the skin juice, gel juice, and flower of Aloe vera tissues affected by fertilizer treatment

Organic and Medicinal Chemistry Letters

Table 1 Yield, molecular weight distribution, and sugar composition (mol%) of A. vera polysaccharides

	Gel juice polysaccharides				Skin juice polysaccharides				Flower polysaccharides
	GN1	GN2	GA1	GA2	SN1	SA1	SA2	SA3	FN1	FA1	FA2
Yield (%)^a	29.2	5.1	17.6	3.7	0.48	0.06	0.02	0.02	2.65	0.59	13.2
Molecular weight distribution (kDa)	230 to 3,340	115 to 207	15 to 110	5 to 13	2 to 59	440-5,575	90 to 290	9 to 52	21 to 108	45 to 125	5 to 30
Molecular weight of each peak (kDa)	690	171	40	9	12	1925	156	31	56	73	21
Sugar composition^b
Rhamnose	-	-	-	ND	-	-	ND	ND	2.0	ND	11.5
Xylose	-	-	1.2	ND	-	8.1	ND	ND	3.1	ND	7.6
Mannose	15.1	13.5	2.0	ND	6.2	21.7	ND	ND	22.9	ND	14.3
Glucose	80	81.2	30.1	ND	29.7	27.0	ND	ND	40.3	ND	20.1
Galactose	3.2	5.1	36.5	ND	62.8	10.90	ND	ND	30	ND	27.8
Glucuronic acid^c	-	-	27.5	ND	-	30.2	ND	ND	-	ND	16.1

Yield, molecular weight distribution and sugar composition (mol%) of the polysaccharides from the skin juice, gel juice, and flower of A. vera purified after gel permeation chromatography. Hyphen denotes values that were not detected. ND, not determined. ^aPercentage weight of crude polysaccharides recovered. ^bPercentage mol of total carbohydrate content. ^cPolysaccharide was hydrolyzed in 2 M sulfuric acid at 100 °C for 8 h. Absorbance at 530 nm was measured according to the m-hydroxydiphenyl method, and the amount was calculated as galacturonic acid.