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Table 1 Validation summary

From: Method development and validation of potent pyrimidine derivative by UV-VIS spectrophotometer

Serial number Parameters Acceptance criteria Observation
01 Precision   
(a) System precision %RSD NMT 1.5% 0.0968
(b) Method precision %RSD NMT 1.5% 0.27995
02 Specificity No considerable absorbance of any other component of formulation at λmax of analyte or at detection wavelength No absorbance observed at 275 nm
03 Accuracy (by recovery)  
% Recovery 100% ± 2% 100.12%
%RSD NMT 1.5% 1.1777%
% Deviation from accuracy ±1.5% 080%: +01.46
  100%: −00.77
  120%: −00.32
04 Linearity Coefficient of correlation r2: 0.997
(r2) NLT 0.998
05 Ruggedness %RSD: NMT 1.5% %RSD: 0.1572
06 Robustness %RSD: NMT 1.5% Original condition: +0.72
Changed condition: −0.82
07 Limit of detection and limit of quantitation LOD 145.2 mg
LOQ 440.00 mg
  1. NMT, not more than; NLT, not less than.