Method development and validation of potent pyrimidine derivative by UV-VIS spectrophotometer

Organic and Medicinal Chemistry Letters

Table 1 Validation summary

Serial number	Parameters	Acceptance criteria	Observation
01	Precision
	(a) System precision %RSD	NMT 1.5%	0.0968
	(b) Method precision %RSD	NMT 1.5%	0.27995
02	Specificity	No considerable absorbance of any other component of formulation at λ_max of analyte or at detection wavelength	No absorbance observed at 275 nm
03	Accuracy (by recovery)
	% Recovery	100% ± 2%	100.12%
	%RSD	NMT 1.5%	1.1777%
	% Deviation from accuracy	±1.5%	080%: +01.46
			100%: −00.77
			120%: −00.32
04	Linearity	Coefficient of correlation	r²: 0.997
04	Linearity	(r²) NLT 0.998	r²: 0.997
05	Ruggedness	%RSD: NMT 1.5%	%RSD: 0.1572
06	Robustness	%RSD: NMT 1.5%	Original condition: +0.72
06	Robustness	%RSD: NMT 1.5%	Changed condition: −0.82
07	Limit of detection and limit of quantitation	LOD	145.2 mg
07	Limit of detection and limit of quantitation	LOQ	440.00 mg