- Original article
- Open Access
Pharmacophore generation and atom-based 3D-QSAR of N-iso-propyl pyrrole-based derivatives as HMG-CoA reductase inhibitors
© Teli and K et al.; licensee Springer. 2012
- Received: 5 January 2012
- Accepted: 10 April 2012
- Published: 2 July 2012
Coronary heart disease continues to be the leading cause of mortality and a significant cause of morbidity and account for nearly 30% of all deaths each year worldwide. High levels of cholesterol are an important risk factor for coronary heart disease. The blockage of 3-hydroxy-3-methylglutaryl-coenzyme A (HMG-CoA) reductase activity by small molecule inhibitors has been shown to inhibit hypercholesterolemia. Herein, we describe the development of effective and robust pharmacophore model and the structure–activity relationship studies of 43N-iso-propyl pyrrole-based derivatives previously reported for HMG-CoA reductase inhibition.
A 5-point pharmacophore model was developed and the generated pharmacophore model was used to derive a predictive atom-based 3D quantitative structure–activity relationship analysis (3D-QSAR) model for the studied dataset. The obtained 3D-QSAR model has an excellent correlation coefficient value (r2 = 0.96) along with good statistical significance as shown by high Fisher ratio (F = 143.2). The model also exhibited good predictive power confirmed by the high value of cross validated correlation coefficient (q2 = 0.672). Further, pharmacophoric model was employed for virtual screening to identify four potential HMG-CoA reductase inhibitors.
The QSAR model suggests that electron-withdrawing character is crucial for the HMG-CoA reductase inhibitory activity. In addition to the electron-withdrawing character, hydrogen bond--donating groups, hydrophobic and negative ionic groups positively contribute to the HMG-CoA reductase inhibition. These findings provide a set of guidelines for designing compounds with better HMG-CoA reductase inhibitory potential.
- HMG-CoA reductase
- N-iso-propyl pyrrole-based derivatives
High levels of cholesterol are linked to atherosclerosis, which is the accumulation of cholesterol-rich fatty deposits in arteries. This can cause arteries to narrow or become blocked, slowing or stopping the flow of blood to vital organs, especially the heart, brain and kidney [1–5]. When atherosclerosis blocks arteries that supply blood to the brain, it can cause an ischemic stroke. Atherosclerotic heart disease is the leading cause of death, accounting for one-third of all deaths and atherosclerotic interference with blood supply to the brain (ischemic stroke) is the third most common cause of death after cancer [6–9]. The biosynthetic pathway for cholesterol involves more than 25 different enzymes and the major rate-limiting step in this pathway is regulated by the 3-hydroxy- 3-methylglutaryl Coenzyme A (HMG-CoA) reductase. Inhibition of HMG-CoA reductase leads to a decrease in intrahepatic cholesterol concentration [10–14].
In recent years, several successful strategies for the inhibition of cholesterol biosynthesis have effectively been demonstrated in preclinical and clinical settings. For examples, ezetimibe and statins have been approved for the treatment of hypercholesterolemia [15–17]. Up to now, there are still a lot of researches focusing on the development of novel inhibitors of HMG-CoA reductase [16, 17]. Recently, a novel series of HMG-CoA reductase inhibitors, which can selectively inhibit HMG-CoA reductase with high inhibitory activities, has been reported by Pfefferkorn et al. .
Keeping the therapeutic significance of this class of inhibitors in mind and our continuing interest in the development of HMG-CoA reductase inhibitors, a 3D quantitative structure–activity relationship analysis (3D-QSAR) was proposed on the series of N-iso-propyl pyrrole-based derivatives reported for HMG-CoA reductase inhibitory activity . The compounds reported were used for the purpose of 3D-QSAR modeling since they have a common scaffold and the biological assays have been carried out in the same lab.
3D-QSAR is widely used in the lead optimization stage and provides valuable insights about ligand–protein interactions [19, 20]. In order to establish relationship between the spatial 3D pharmacophoric features and HMG-CoA reductase inhibitors activity of a group of N-iso-propyl pyrrole-based derivatives, a 3D-QSAR analysis was carried out on the combined dataset employing PHASE module of Schrodinger molecular modeling software .
In this study, we have developed a quantitative pharmacophore model based on HMG-CoA reductase inhibitors collected from the same laboratory . The best quantitative model was used as a 3D search query for screening the ZINC database to identify new inhibitors of HMG-CoA reductase. Once identified, the candidate compounds were subsequently subjected to filtration by molecular docking.
A set of 43N-iso-propyl pyrrole-based derivatives with well-defined HMG-CoA reductase inhibitory activity was used for the QSAR analysis . In vitro inhibitory concentrations (IC50) of the molecules against HMG-CoA reductase were converted into corresponding pIC50 [−log(IC50)] and were used as dependent variables in the QSAR calculations (Additional file 1).
PHASE 3.0 module of Schrodinger molecular modeling software was used to generate pharmacophore models . In QSAR studies, appropriate conformation (lowest energy) of the compound is required for accurate calculation of 3D descriptors. All the 43 compounds were sketched by Maestro. Further, geometry optimization was carried out using the semi-empirical OPLS_2005 force field. All the molecules were divided into a training set and test set to maintain the structure and activity diversity in both the sets for QSAR model and pharmacophore generation and validation.
PHASE incorporates a structure cleaning step utilizing LigPrep, which attaches hydrogens, converts 2D structures to 3D, generates stereoisomers and neutralizes charged structures or determines the most probable ionization state at a user-defined pH. It also allows for the importation of 3D structures prepared outside its own workflow. Because one does not generally know the structure that a given molecule will adopt if and when it binds to a target protein, it is customary to represent each molecule as a series of 3D structures that sample the thermally accessible conformational states. For the purposes of pharmacophore model development, PHASE provides two built-in approaches, both of which employ the MacroModel conformational search engine. Since most of the studied ligands were flexible, all possible conformations for a ligand were generated to consider a range of conformations in order to increase the chances of finding the most active conformer close to the bound structure. Accordingly, the prepared ligands were subjected to conformational analysis using MonteCarlo Multiple Minimum method implemented in the Schrodinger software.
The ligands were assigned as active and inactive by giving an appropriate activity threshold value. The threshold value was 6 for active legend and 5 for inactive ligand. The activity threshold value was selected on the basis of dataset activity distribution (4.427–6.699) and the active ligands were chosen to derive a set of suitable pharmacophores. The so-prepared ligands were used for generating common pharmacophore and QSAR model building.
Creation of pharmacophore sites
The chemical features of all ligands were defined by six pharmacophorics features: H-bond acceptor (A), H-bond donor (D), hydrophobic group (H), negatively charged group (N), positively charged group (P), and aromatic ring (R). An active analog approach was used to identify common pharmacophore hypotheses (CPHs), in which common pharmacophores were culled from the conformations of the set of active ligands using a tree-based partitioning technique that groups together similar pharmacophores according to their intersite distances .
Scoring pharmacophores with respect to active and inactive ligands
The resulting pharmacophores were then scored and ranked. The scoring was done to identify the best candidate hypothesis, which provided an overall ranking of all the hypotheses. The scoring algorithm included the contributions from the alignment of site points and vectors, volume overlap, selectivity, number of ligands matched, relative conformational energy, and activity .
Perceiving common pharmacophores
Score of different parameters of the AANRR hypothesis
Building 3D-QSAR models
QSAR modeling was carried out using the selected hypothesis by dividing the dataset in to training set (70%) and test set (30%) in a random manner. PHASE presents two options for alignment of 3D structure of molecules: the pharmacophore based alignment and the atom-based alignment [21, 23]. In this study, we have used an atom-based QSAR model, which is more useful in explaining the structure–activity relationship. In atom-based QSAR, a molecule is treated as a set of overlapping van der Waal’s spheres. Each atom (and hence each sphere) is placed into one of six categories according to a simple set of rules: hydrogens attached to polar atoms are classified as hydrogen bond donors (D); carbons, halogens, and C–H hydrogens are classified as hydrophobic/non-polar (H); atoms with an explicit negative ionic charge are classified as negative ionic (N); atoms with an explicit positive ionic charge are classified as positive ionic (P); non-ionic nitrogen and oxygen are classified as electron-withdrawing (W); and all other types of atoms are classified as miscellaneous (X).
For the purpose of QSAR development, van der Waal’s models of the aligned training set molecules were placed in a regular grid of cubes, with each cube allotted zero or more ‘bits’ to account for different types of atoms in the training set that occupy the cube. This representation gives rise to binary-valued occupation patterns that can be used as independent variables to create partial least-squares (PLS) QSAR models. Atom-based QSAR models were generated for the selected hypothesis using the 31-member training set using a grid spacing of 1.0 Å. The best QSAR model was validated by predicting activities of the 12 test set compounds. A four-component (PLS factor) model with good statistics was obtained for the dataset whereas the maximum number of PLS factors in each model can be 1/5 of the total number of training set molecules. Further increase in the number of PLS factors did not improve the model statistics or predictive ability .
The HMG-CoA reductase (PDB ID: 2R4F) was prepared using the Protein Preparation Wizard of the Schrödinger suite. H-atoms were added to the protein, including the protons necessary to define the correct ionization and tautomeric states of amino acid residues such as Asp, Ser, Glu, Arg, and His. The missing side chains of residues were corrected using Build interface incorporated in Maestro. For each structure, minimization was carried out with the Impact Refinement module, using the OPLS-2005 force field to alleviate steric clashes that may exist in the structures. The minimization was terminated when the energy converged or the Root Mean Square Deviation (RMSD) reached a maximum cutoff of 0.30 Å . After protein preparation, receptor grid was set up, which was generated by employing the Receptor Grid Generation panel. Since this protein was associated with ligand, the ligand was selected to define the position and size of the active site.
AANRR hypothesis was used to search a 3D database for structures that match the pharmacophoric features of the model. Virtual screening was carried out using ZincPharmer that uses the pharmacophore to efficiently search the ZINC database of fixed conformers for pharmacophore matches [25, 26]. To accomplish the best 3D similarity search, we used constraints that included maximum of 0.7 RMSD, obeying 10 rotatable bond cutoff and molecular weight range of 180–500 Dalton. A molecule which fits well with the pharmacophoric features of the AANRR hypothesis was retrieved as a hit.
All the hits with good RMSD value were enlisted and were incorporated to Maestro. Hits were subjected to hydrogen additions, removal of salt, ionization and generation of low-energy ring conformations using LigPrep. The chiralities of the original compounds were preserved. Finally, the low-energy 3D structures of all compounds were produced. The virtual screening workflow (VSW) in Maestro was used to dock and to score the lead-like compounds. In the first step, Glide was run in high-throughput virtual screen mode. 10% of the top-scoring ligands were kept to go onto the next, Glide Single Precision (SP), stage. The top-scoring 10% leads from GlideSP were retained and docked with Glide XP. All the Glide protocols were run using default parameters. An extensive search was carried out for generating all possible conformations. Minimization cycle for conjugate gradient and steepest descent minimizations were used with default value of 0.05 Å for initial step size and 1.0 Å for maximum step size. In convergence criteria for the minimization, both the energy change criteria and gradient criteria were used with default values of 10-7 and 0.001 kcal/mol, respectively. Then, all conformations were considered for docking. During the docking process, the Glide-score was used to select the best conformation for each ligand.
PLS statistical parameters of the selected 3D-QSAR model
For the generation of pharmacophore model, we have considered 12 compounds having activity > 6 against HMG-CoA reductase as active as they contain important structural features crucial for binding to the receptors binding site. We used four minimum sites and five maximum sites to have optimum combination of sites or features common to the most active compounds. One hundred and two common pharmacophore models were generated with different combination of variants in which all models were considered for further QSAR generation; the results were illustrated in (Additional file 2). Among these pharmacophores, the models which are showing the superior alignment with active compounds were identified by mapping to them and calculating the survival score. The survival scoring function identifies the best candidate hypothesis from the generated models and provides an overall ranking of all the hypotheses. The scoring algorithm includes contributions from the alignment of site points and vectors, volume overlap, selectivity, number of ligands matched, relative conformational energy, and activity. However, these pharmacophore models should also discriminate between the active (most active) and inactive (less active) molecules (Table 1). It is true that the hypothesis is incomplete if it lacks either a critical site that explains the binding or information on what prevents inactive ligands from binding. To identify the pharmacophore models with more active and less inactive features among these models, they were mapped to inactive compounds and scored. If inactive ligands score well, the hypothesis could be invalid because it does not discriminate between active and inactive ligands. Therefore, adjusted survival score was calculated by subtracting the inactive score from survival score of these pharmacophores (Additional file 2). Finally the models with maximum adjusted survival score and lowest relative conformational energy ware selected for generating pharmacophore (atom)-based alignment of HMG-CoA reductase inhibitors and model AANRR has been chosen because it produced good predictive power above other models. The special arrangement of features along with their distance present in five-featured pharmacophore, AANRR, was shown in Figure 1a. As depicted in the figure, among the two ring aromatic features, one feature is mapped to the pyrrole ring of all 12 active inhibitors and another on benzene side chain attached to pyrrole ring. The both hydrogen bond acceptor and negative ionic features are mapped to (the hydroxyl groups and carboxyl group) on N-iso-propyl side chain substituted on pyrrole ring.
Fitness and predicted activity data for test and training set of compounds
A 3D-QSAR analysis was performed on the series of N-iso-propyl pyrrole-based derivatives to understand the effect of spatial arrangement of structural features on their HMG-CoA reductase inhibition. The large value of F (143.2) indicates a statistically significant regression model, which is also supported by the small value of the variance ratio (P), an indication of a high degree of confidence. Further, small values of standard deviation (0.137) of the regression and RMSE (RMSE = 0.296) makes an obvious implication that the data used for model generation are best for the QSAR analysis. Validity of the model can be expressed by cross-validated correlation coefficient (q2 = 0.672) that was obtained by leave one out or leave N out method. The q2 is more reliable and robust statistical parameter than r2 because it is obtained by external validation method by dividing the dataset into training and test set.
Visual analysis of Figure 3a demonstrates that the presence of the blue cubes at the A2, A3, N10 (N-iso-propyl side chain), cyano group, and amide group (carbamoyl side chain) attached to pyrrole ring is pointing out the positive potential of electron withdrawing characteristic of the molecules and is requisite for the activity at this particular place. It can be suggested that addition of appropriate electron withdrawing groups at the A2, A3, cyano, and amide (carbamoyl side chain) sites will append the HMG-CoA reductase inhibition, whereas the addition of electron-withdrawing groups near carboxy group attached to pyrrole ring position will contribute to decreased receptor binding, which in turn will result in lower potency of compounds because red color cubes are for negative effect of electron-withdrawing group to that particular place. Figure 3b illustrates that H-bond donor characteristic is necessary at A2 and A3 (N-iso-propyl side chain) present on the pyrrole ring. The red cubes at the benzene ring attached to the amide side chain of pyrrole ring demonstrate a negative potential of H-bond donor at that position on the compounds. Figure 3c demonstrates the effect of hydrophobic groups on HMG-CoA reductase inhibition. It can be deduced from the figure that hydrophobic groups are well tolerated near cyano benzene ring on carbamoyl side chain (blue cubes), while the substitution of hydrophobic groups in between amide (carbamoyl side chain) and cyano benzene ring site are unacceptable (red cubes) or may hinder the binding of the molecules to the receptor active site and will result in decreased HMG-CoA reductase inhibition. Further, Figure 3d showed combined effect of all the features. The presence of hydroxyl groups (N-iso-propyl side chain), cyano group, and amide group (carbamoyl side chain) will have the positive effect (blue cubes).
One efficient approach to drug discovery is the virtual screening of the molecular libraries. Pharmacophore-based database searching is considered as a type of ligand-based virtual screening, which can efficiently be used to find novel and potential leads for further development. A potent pharmacophore model possesses the chemical functionalities responsible for bioactivities of potential drugs; therefore, it can be used to perform a database search by serving as a 3D query. The pharmacophore AANRR was used as a 3D structural query for retrieving potent molecules from the ZINC chemical database through ZincPharmer. To avoid possible incorrect predictions, the molecular weight cutoff of 180–500 Dalton range, RMSD of 0.7, and cutoff limit of 10 rotatable bonds filters were applied. Altogether, 3D structural query retrieved 17,283 compounds from the ZINC database.
Drug likeness analysis
The ZINC database was evaluated for drug-likeness of the lead molecules by assessing their physicochemical properties and by applying Lipinski’s rule of five. Their molecular weights were <500 Daltons with <5H-bond donors, <10H-bond acceptors and a log p of <5; these properties were well within the acceptable range of the Lipinski rule for drug-like molecules.
ADME properties of selected hits with the docking score
% Oral absorption e
Docking was carried out to increase the power of the pharmacophore-based screening to discriminate between the active and inactive ligands (that could adopt poses to accomplish the pharmacophore features). The docking was especially useful during the second step of the VS workflow for the identification of molecules having at least one conformer that irrespective of its initial spatial orientation, can fit into the pharmacophore (because docking can be used to exclude those inactive molecules from the VS having at least one conformer, which matches the sites in the pharmacophore but is sterically hindered by other residues in the active site).
A ligand-based pharmacophore model was generated for the series of N-iso-propyl pyrrole-based derivatives with HMG-CoA reductase inhibitory activity to reveal the structural features responsible for biological activity. With the help of pharmacophore-based alignment of HMG-CoA reductase inhibitors, a meaningful 3D-QSAR was derived to identify and how three-dimensional arrangements of various substituents will affect the HMG-CoA reductase inhibition. The selected model as shown by the correlation statistics and predictive statistics is very much significant to draw unambiguous inferences. Further, the generated 3D-QSAR model also explains how and at what extent electron withdrawing, hydrophobic and H-Donor moieties in the molecular structure influences HMG-CoA reductase inhibition showed by the title compounds. The model shows that HMG-CoA reductase inhibition can be increased, if the electron-withdrawing character near N-iso-propyl side chains is supplemented by appropriate functional groups and by the incorporation of H-donor, hydrophobic, and positive ionic groups at specific positions in the molecules. Finally, four potential hits with good fitness value and predicted activity were identified by virtual screening and docking, whose activity can be further improved with the help of this QSAR model. This study provides a set of guidelines which will greatly help in designing the newer and more potent HMG-CoA reductase inhibitors of the N-iso-propyl pyrrole-based scaffold.
- Ballantyne CM, Houri J, Notarbartolo A, Melani L, Lipka LJ, Suresh R, Sun S, LeBeaut AP, Sager PT, Veltri EP: Effect of ezetimibe coadministered with atorvastatin in 628 patients with primary hypercholesterolemia: a prospective, randomized, double-blind trial. Circulation 2003, 107: 2409–2415. 10.1161/01.CIR.0000068312.21969.C8View ArticleGoogle Scholar
- Bays HE, Moore PB, Drehobl MA, Rosenblatt S, Toth PD, Dujovne CA, Knopp RH, Lipka LJ, Lebeaut AP, Yang B, Mellars LE, Cuffie-Jackson C, Veltri EP: Effectiveness and tolerability of ezetimibe in patients with primary hypercholesterolemia: pooled analysis of two phase II studies. Clin Ther 2001, 23: 1209–1230. 10.1016/S0149-2918(01)80102-8View ArticleGoogle Scholar
- Bergström H, Andersson P: Statins and polyneuropathy. Lakartidningen 2008, 105: 826–827.Google Scholar
- Clader JW: The discovery of ezetimibe: a view from outside the receptor. J Med Chem 2004, 47: 1–9. 10.1021/jm030283gView ArticleGoogle Scholar
- Davidson MH, McGarry T, Bettis R, Melani L, Lipka LJ, LeBeaut AP, Suresh R, Sun S, Veltri EP: Ezetimibe coadministered with simvastatin in patients with primary hypercholesterolemia. J Am Coll Cardiol 2002, 40: 2125–2134. 10.1016/S0735-1097(02)02610-4View ArticleGoogle Scholar
- Davis HR Jr, Zhu LJ, Hoos LM, Tetzloff G, Maguire M, Liu J, Yao X, Iyer SP, Lam MH, Lund EG, Detmers PA, Graziano MP, Altmann SW: Niemann-Pick C1 Like 1 (NPC1L1) is the intestinal phytosterol and cholesterol transporter and a key modulator of whole-body cholesterol homeostasis. J Biol Chem 2004, 279: 33586–33592. 10.1074/jbc.M405817200View ArticleGoogle Scholar
- Soliemani A, Nikoueinejad H, Tabatabaizade M, Mianehsaz E, Tamadon M: Effect of hydroxymethylglutaryl-coa reductase inhibitors on low-density lipoprotein cholesterol, interleukin-6, and high-sensitivity C-reactive protein in end-stage renal disease. Iran J Kidney Dis 2011, 5: 29–33.Google Scholar
- Notarnicola M, Messa C, Refolo MG, Tutino V, Miccolis A, Caruso MG: Synergic effect of eicosapentaenoic acid and lovastatin on gene expression of HMGCoA reductase and LDL receptor in cultured HepG2 cells. Lipids Health Dis 2010, 9: 135. 10.1186/1476-511X-9-135View ArticleGoogle Scholar
- Armitage J, Bowman L, Wallendszus K, Bulbulia R, Rahimi K, Haynes R, Parish S, Peto R, Collins R: Intensive lowering of LDL cholesterol with 80 mg versus 20 mg simvastatin daily in 12,064 survivors of myocardial infarction: a double-blind randomised trial. Study of the effectiveness of additional reductions in cholesterol and homocysteine. Lancet 2010, 376: 1658–1669. 10.1016/S0140-6736(10)60310-8View ArticleGoogle Scholar
- Lardizabal JA, Deedwania PC: Benefits of statin therapy and compliance in high risk cardiovascular patients. Vasc Health Risk Manag 2010, 6: 843–853.Google Scholar
- Gomes ME, Tack CJ, Verheugt FW, Smits P, Lenders JW: Sympathoinhibition by atorvastatin in hypertensive patients. Circ J 2010, 74: 2622–2626. 10.1253/circj.CJ-10-0427View ArticleGoogle Scholar
- Inadera H, Hamazaki T: Cholesterol controversy: cutoff point of low-density lipoprotein cholesterol level in Guidelines by Japan Atherosclerosis Society. Nippon Eiseigaku Zasshi 2010, 65: 506–515. 10.1265/jjh.65.506View ArticleGoogle Scholar
- Koniari IC, Tsamis K, Apostolakis E: Simvastatin inhibits aortic valve calcification in hypercholesterolemic rabbits. Interact Cardiovasc Thorac Surg 2010, 11: 301–302. 10.1510/icvts.2009.230920AView ArticleGoogle Scholar
- Ghirlanda G, Oradei A, Manto A, Lippa S, Uccioli L, Caputo S, Greco A, Littarru G: Evidence of plasma CoQ10-lowering effect by HMG-CoA reductase inhibitors: a double-blind, placebo-controlled study. J Clin Pharmacol 1993, 33: 226–229.View ArticleGoogle Scholar
- Grundy SM: Absorption and metabolism of dietary cholesterol. Annu Rev Nutr 1983, 3: 71–96. 10.1146/annurev.nu.03.070183.000443View ArticleGoogle Scholar
- Knopp RH, Gitter H, Truitt T, Bays H, Manion CV, Lipka LJ, LeBeaut AP, Suresh R, Yang B, Veltri EP: Effects of ezetimibe, a new cholesterol absorption inhibitor, on plasma lipids in patients with primary hypercholesterolemia. Eur Heart J 2003, 24: 729–741.View ArticleGoogle Scholar
- Rajanikant GK, Zemke D, Kassab M, Majid A: The therapeutic potential of statins in neurological disorders. Cur Med Chem 2007, 14: 103–112. 10.2174/092986707779313462View ArticleGoogle Scholar
- Pfefferkorn JA, Song Y, Sun KL, Miller SR, Trivedi BK, Choi C, Sorenson RJ, Bratton LD, Unangst PC, Larsen SD, Poel TJ, Cheng XM, Lee C, Erasga N, Auerbach B, Askew V, Dillon L, Hanselman JC, Lin Z, Lu G, Robertson A, Olsen K, Mertz T, Sekerke C, Pavlovsky A, Harris MS, Bainbridge G, Caspers N, Chen H, Eberstadt M: Design and synthesis of hepatoselective, pyrrole-based HMG-CoA reductase inhibitors. Bioorg Med Chem Lett 2007, 17: 4538–4544. 10.1016/j.bmcl.2007.05.096View ArticleGoogle Scholar
- Mahipal TOP, Karthikeyan C, Moorthy NS, Trivedi P: 3D QSAR of aminophenyl benzamide derivatives as histone deacetylase inhibitors. Med Chem 2010, 6: 277–285. 10.2174/157340610793358846View ArticleGoogle Scholar
- Leone S, Mutti C, Kazantsev A, Sturlese M, Moro S, Cattaneo E, Rigamonti D, Contini A: SAR and QSAR study on 2-aminothiazole derivatives, modulators of transcriptional repression in Huntington’s disease. Bioorg Med Chem 2008, 16: 5695–5703. 10.1016/j.bmc.2008.03.067View ArticleGoogle Scholar
- Dixon SL, Smondyrev AM, Rao S: PHASE: A Novel Approach to Pharmacophore Modeling and 3D Database Searching. Chem Biol Drug Des 2006, 67: 370–372. 10.1111/j.1747-0285.2006.00384.xView ArticleGoogle Scholar
- Dixon SL, Smondyrev AM, Knoll EH, Rao SN, Shaw DE, Friesner RA: PHASE: a new engine for pharmacophore perception, 3D QSAR model development, and 3D database screening: 1. Methodology and preliminary results. J Comput Aided Mol Des 2006, 20: 647–671. 10.1007/s10822-006-9087-6View ArticleGoogle Scholar
- Teli MK, Rajanikant GK: Pharmacophore generation and atom-based 3D-QSAR of novel quinoline-3-carbonitrile derivatives as Tpl2 kinase inhibitors. J Enzyme Inhib Med Chem 2011, 26: 1–13. 10.3109/14756360903524304View ArticleGoogle Scholar
- Teli MK, Rajanikant GK: Identification of novel potential HIF-prolyl hydroxylase inhibitors by in silico screening. Mol Divers 2012, 16: 193–202. 10.1007/s11030-011-9338-xView ArticleGoogle Scholar
- Koes DR, Camacho CJ: Pharmer: efficient and exact pharmacophore search. J Chem Inf Model 2011, 51: 1307–1314. 10.1021/ci200097mView ArticleGoogle Scholar
- Irwin JJ, Shoichet BK: ZINC—a free database of commercially available compounds for virtual screening. J Chem Inf Model 2005, 45: 177–182. 10.1021/ci049714+View ArticleGoogle Scholar
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